EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.4.53 | Co2+ |
the 3',5'-phosphodiesterase enzyme activities of PdeA and PdeB are stimulated 3.2fold and 1.98old, respectively, by 0.05 mM Co2+ at pH 8.0 in 50 mM Tris-HCl buffer |
692369 |
| 3.1.4.53 | Co2+ |
the enzyme is able to use Mg2+, Co2+, and Mn2+, but not Zn2+ |
685054 |
| 3.1.4.53 | Fe2+ |
Fe2+ is required for enzyme activity. The catalytic mechanism for CpdA utilizes a Fe3+-Fe2+ center. Addition of 0.01 mM FeCl2, as a source of Fe2+, results in an 2fold stimulation of CpdA activity |
712298 |
| 3.1.4.53 | Fe2+ |
iron and conserved residues are essential for CpdA activity |
712298 |
| 3.1.4.53 | Fe2+ |
required |
750557 |
| 3.1.4.53 | Mg2+ |
activity was higher in the presence of Mn2+ than Mg2+ as cofactor |
684955 |
| 3.1.4.53 | Mg2+ |
required |
670189, 681819 |
| 3.1.4.53 | Mg2+ |
required for activity and stability |
681819 |
| 3.1.4.53 | Mg2+ |
stimulates |
688885 |
| 3.1.4.53 | Mg2+ |
the concentration-response curve for Mg2+ is biphasic and ranges from 0.1 to 100 mM. Phosphorylation of PDE-4D3 by protein kinase A produces a monophasic Mg2+ response curve |
689067 |
