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EC Number	Metals/Ions	Commentary	Reference
2.7.2.2	Bivalent cation	required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+	642219
2.7.2.2	Co2+	bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+	642219
2.7.2.2	Fe2+	bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+	642219
2.7.2.2	Mg2+	-	642219
2.7.2.2	Mg2+	10 mM used in assay conditions	760396
2.7.2.2	Mg2+	bivalent cation required, Mg2+ is the most commonly used, enzyme from Streptococcus lactis is also fully active with Mn2+ and less so with Fe2+ and Co2+	642219
2.7.2.2	Mg2+	Mn2+ is as effective as Mg2+	642221
2.7.2.2	Mg2+	Mn2+ or Mg2+ required, higher activity with Mn2+	642220
2.7.2.2	Mg2+	required	642217, 642221, 735216, 738607, 739466
2.7.2.2	Mn2+	-	642219

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Release 2023.1 (January 2023)