EC Number   |
Metals/Ions |
Reference |
|---|
    2.5.1.84 | Co2+ |
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme |
637962 |
| 2.5.1.84 | Mg2+ |
enzyme requires Mg2+, variable product specificity depending on Mg2+ concentration |
637959 |
| 2.5.1.84 | Mg2+ |
Mg2+ is absolutely required for activity, Mn2+ is less effective. The chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and c40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds |
637961 |
| 2.5.1.84 | Mg2+ |
no activity in absence of divalent metal ion, activation with 2 mM Mg2+ is 50% of that obtained with Mn2+ |
637967 |
| 2.5.1.84 | Mg2+ |
stimulates activity |
637966 |
| 2.5.1.84 | Mg2+ |
the concentration of the complex between farnesyl diphosphate and Mg2+, the priming substrate for this synthase, does not affect the product distribution, provided that the concentration of isopentenyl diphosphate/Mg2+ is maintained at a certain level. The level of isopentenyl diphosphate/Mg2+ is decisive in affecting the chain length distribution of the products of the prenyltransferase reaction |
637963 |
| 2.5.1.84 | Mg2+ |
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme |
637962 |
| 2.5.1.84 | Mn2+ |
Mg2+ is absolutely required for activity, Mn2+ is less effective |
637961 |
| 2.5.1.84 | Mn2+ |
no activity in absence of divalent metal ion, 0.1 mM Mn2+ is required for optimal activity |
637967 |
| 2.5.1.84 | Mn2+ |
with MgCl2 nonaprenyl diphosphate is the longest product, with MnCl2 or CoCl2, the longest products are C50 and C55, respectively, partially purified enzyme |
637962 |
