EC Number   |
Metals/Ions |
Reference |
|---|
    1.8.1.4 | Cd2+ |
highly, specifically stimulating for ubiquinone reduction, optimal at 0.5 mM |
658600 |
| 1.8.1.4 | Fe |
amino acid sequence homology indicates the presence of a Fe-containing rubredoxin-like domain. The amount of iron is determined to be 1.1 molecule per rDiaA molecule by ICP atomic emission spectroscopy |
702739 |
| 1.8.1.4 | KCl |
maximal enzymatic activity at 2 to 3 M KCl |
727729 |
| 1.8.1.4 | Mg2+ |
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors |
394001 |
| 1.8.1.4 | more |
1 mM Na+, Ca2+, Mg2+, Mn2+, Fe3+, Zn2+, Pb2+, and Hg2+ do not enhance enzyme activity |
691392 |
| 1.8.1.4 | NaCl |
completely inactive in absence of NaCl |
393978 |
| 1.8.1.4 | Zn2+ |
highly activating ubiquinone reduction, up to 0.5 mM |
659866 |
| 1.8.1.4 | Zn2+ |
highly stimulating for ubiquinone reduction |
658231 |
| 1.8.1.4 | Zn2+ |
highly, specifically stimulating for ubiquinone reduction, optimal at 0.5 mM, Zn2+ slightly elevates the pH-optimum of the enzyme |
658600 |
