EC Number   |
Metals/Ions |
Reference |
|---|
   1.13.12.24 | Ca2+ |
absolutely required |
744887 |
| 1.13.12.24 | Ca2+ |
Ca2+-regulated photoprotein |
765520, 765521, 765527 |
| 1.13.12.24 | Ca2+ |
calcium-regulated photoprotein |
763804, 764863, 764867, 765523, 765663 |
| 1.13.12.24 | Ca2+ |
Kd value 0.013 mM for wild-type, 0.26 mM for mutant D119A |
744205 |
| 1.13.12.24 | Ca2+ |
required for luminescence reaction |
745437 |
| 1.13.12.24 | Ca2+ |
sequence displays three F-F hand structures that are characteristic for Ca2+-binding sites |
746326 |
| 1.13.12.24 | Ca2+ |
the binding of calcium ion to the photoprotein brings about a conformational change in the protein which results in the chromophore reacting with a protein side chain hydroperoxide to yield a protein bound luciferin hydroperoxide intermediate. This intermediate then decomposes to CO2 and an electronic excited state of the corresponding protein-oxyluciferin monoanion complex which leads to light emission |
744286 |
| 1.13.12.24 | Ca2+ |
when calcium binds at either or both EF hands I and IV the helices of these hands will change their relative orientations |
745878 |
| 1.13.12.24 | Mg2+ |
binding of Mg2+ to aequorin prevents the molecule from aggregating and stabilizes it in the monomeric form. Mg2+ binding induces conformational in the EF-hand loops. There are two Mg2+-binding sites, EF-hands I and III. EF-hand III binds to Mg2+ with higher affinity than EF-hand I, and only EF-hand III seems to be occupied by Mg2+ under physiological conditions |
745232 |
