EC Number |
Metals/Ions |
Reference |
---|
1.13.11.33 | Ca2+ |
- |
639258 |
1.13.11.33 | Ca2+ |
1 mM |
672376 |
1.13.11.33 | Ca2+ |
50% of maximal activity at 0.1 mM Ca2+ in presence of 5 mM EGTA |
639250 |
1.13.11.33 | Ca2+ |
activates reaction with crude extract |
639253 |
1.13.11.33 | Ca2+ |
assay in presence of Ca2+ |
702247 |
1.13.11.33 | Ca2+ |
at 0.005-0.05 mM is required for efficient membrane binding |
659378 |
1.13.11.33 | Ca2+ |
Ca2+ binds to the PLAT domain of 15-LOX-2, resulting in a translocation from the cytosol to the membrane |
741942 |
1.13.11.33 | Ca2+ |
enhances activity, reversible by EGTA, inhibited by phosphatidyl serine and phosphatidyl choline |
639241 |
1.13.11.33 | Ca2+ |
for maximal intracellular activity, 12/15-lipoxygenases require a rise in cytosolic calcium concentration inducing a translocation of the enzyme from the cytosol to cellular membranes |
639258 |
1.13.11.33 | Ca2+ |
in the presence of Ca2+, with or without calcium ionophore, the majority of 15-LO-1 is found in the membrane fraction, although significant amounts are also detected in the supernatant fraction |
703628 |