EC Number   |
Metals/Ions |
Reference |
|---|
    1.13.11.31 | Ca2+ |
1 mM Ca2+-dependent activity |
676096 |
| 1.13.11.31 | Ca2+ |
10 mM, required for activity |
671591 |
| 1.13.11.31 | Ca2+ |
2 mM ATP + 1 mM CaCl2 stimulate approximately 2fold |
395411 |
| 1.13.11.31 | Ca2+ |
not essential for activity, partially purified enzyme is stimulated approximately 2fold in the presence of 0.1-5.0 mM Ca2+ |
395401 |
| 1.13.11.31 | Ca2+ |
stimulates |
395416 |
| 1.13.11.31 | Ca2+ |
the specific activity of recombinant soluble zf12-LOX mutant is increased by nearly 75% (25 nmol/min/mg) in the presence of calcium as compared to that in its absence (7 nmol/min/mg), while for the site-2 mutant, it is 5 nmol/min/mg irrespective of the presence of calcium. Ca2+ decreases the aggregation temperature of soluble zf12-LOX mutant, while secondary structure is maintained. Presence of Ca2+ induces increase in molecular dimensions of soluble zf12-LOX mutant |
741993 |
| 1.13.11.31 | Fe |
- |
706032 |
| 1.13.11.31 | Fe2+ |
- |
702324 |
| 1.13.11.31 | Fe2+ |
0.94 atoms of iron per molecule. Loss of the mass of an iron atom from the protein as the pH is lowered from 5 to 4. The iron goes through acid-induced unfolding accompanied by the appearance of an iron-free intermediate that retains a native conformation |
701926 |
| 1.13.11.31 | Fe2+ |
a non-heme iron in the active side |
742000 |
