EC Number |
Metals/Ions |
Reference |
---|
5.1.99.1 | Co2+ |
0.4 mM, 4fold increase in activity. Presence of Co2+ restores activity after incubation with EDTA |
693174 |
5.1.99.1 | Co2+ |
activates |
726729 |
5.1.99.1 | Mg2+ |
activates |
726729 |
5.1.99.1 | Ni2+ |
activates |
726729 |
5.1.99.1 | more |
enzyme does not require tightly bound metal ions for activity, they are not released even after extensive dialysis at pH 2.4 |
2437 |
5.1.99.1 | Co2+ |
enzyme is metal-dependent enzyme, in the active site, metal binding site binds the coenzyme Co2+ |
706660 |
5.1.99.1 | more |
His12, Gln65, His91 and Glu141 provide a binding site for a divalent metal ion, as shown by binding of Co2+ |
653930 |
5.1.99.1 | Co2+ |
increases activity |
2437, 2442 |
5.1.99.1 | Mn2+ |
increases activity to a smaller extent than Co2+ |
2437 |
5.1.99.1 | Ni2+ |
increases activity to a smaller extent than Co2+ |
2437 |