EC Number |
Metals/Ions |
Reference |
---|
3.1.1.96 | KCl |
200 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr |
725371 |
3.1.1.96 | KCl |
the initial rate of D-Tyr-tRNATyr hydrolysis, in a 20 mM Tris-HCl buffer (pH 7.8) containing 0.1 mM EDTA, is increased 34-fold upon the addition of 50 mM KCl |
725336 |
3.1.1.96 | Mg2+ |
markedly improves initial rate of D-Tyr-tRNATyr hydrolysis. Optimal concentration: 16 mM |
725371 |
3.1.1.96 | MgCl2 |
the initial rate of D-Tyr-tRNATyr hydrolysis, in a 20 mM Tris-HCl buffer (pH 7.8) containing 0.1 mM EDTA, is increased 34-fold upon the addition of 50 mM MgCl2 |
725336 |
3.1.1.96 | more |
the zinc association, as revealed by crystal analysis, has no functional significance |
725346 |
3.1.1.96 | Zn2+ |
contains 1 Zn2+ per polypeptide chain |
725386 |
3.1.1.96 | Zn2+ |
contains strongly bound Zn2+ |
725371 |
3.1.1.96 | Zn2+ |
wild-type the enzyme contains two firmly bound Zn2+ |
725371 |
3.1.1.96 | Zn2+ |
zinc ions are essential to the activity of the protein |
726062 |