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Results 1 - 9 of 9
EC Number
Metals/Ions
Commentary
Reference
KCl
200 mM, markedly stimulates hydrolysis of D-Tyr-tRNATyr
KCl
the initial rate of D-Tyr-tRNATyr hydrolysis, in a 20 mM Tris-HCl buffer (pH 7.8) containing 0.1 mM EDTA, is increased 3–4-fold upon the addition of 50 mM KCl
Mg2+
markedly improves initial rate of D-Tyr-tRNATyr hydrolysis. Optimal concentration: 16 mM
MgCl2
the initial rate of D-Tyr-tRNATyr hydrolysis, in a 20 mM Tris-HCl buffer (pH 7.8) containing 0.1 mM EDTA, is increased 3–4-fold upon the addition of 50 mM MgCl2
more
the zinc association, as revealed by crystal analysis, has no functional significance
Zn2+
contains 1 Zn2+ per polypeptide chain
Zn2+
contains strongly bound Zn2+
Zn2+
wild-type the enzyme contains two firmly bound Zn2+
Zn2+
zinc ions are essential to the activity of the protein
Results 1 - 9 of 9