EC Number   |
Metals/Ions |
Reference |
|---|
   2.4.1.65 | Mn2+ |
- |
637636, 658008, 693000 |
| 2.4.1.65 | Mn2+ |
absolutely required |
691644 |
| 2.4.1.65 | Mn2+ |
activation above pH 8.0 |
637636 |
| 2.4.1.65 | Mn2+ |
binds the enzyme and increases affinity for the acceptor. One possible functional role of manganese in catalysis can be as an electrophilic catalyst, co-ordinating the negative charges of the phosphate groups of the GDP-Fuc donor and promoting Fuc transfer. A low pH values such role would be played by the proton. Mn2+ leads to 2.7fold activation of SFT3 |
661313 |
| 2.4.1.65 | Zn2+ |
can replace Mn2+ for activation, but shows only half the maximal activity, optimum concentration is 10-15 mM |
660797 |
| 2.4.1.65 | Ca2+ |
can replace Mn2+ for activation, optimum concentration is 10-15 mM |
660797 |
| 2.4.1.65 | Mg2+ |
can replace Mn2+ for activation, optimum concentration is 10-15 mM |
660797 |
| 2.4.1.65 | more |
Cu2+ inactivates the enzyme |
660797 |
| 2.4.1.65 | Mn2+ |
Fuc-T V is Mn2+ dependent |
661341 |
| 2.4.1.65 | more |
FUT10 functions well without MnCl2 |
704613 |
