EC Number   |
Metals/Ions |
Reference |
|---|
   1.1.1.71 | NaCl |
400 mM, 206% of initial activity |
726712 |
| 1.1.1.71 | KCl |
600 mM, 147% of initial activity |
726712 |
| 1.1.1.71 | KCl |
a preference for KCl over NaCl is observed, with best activity at 4 M KCl |
728288 |
| 1.1.1.71 | KCl |
activity increases in the presence of KCl is maintained even at concentration of 3 M |
726712 |
| 1.1.1.71 | NaCl |
activity increases in the presence of NaCl is maintained even at concentration of 4 M |
726712 |
| 1.1.1.71 | NaCl |
activity is increased in the presence of NaCl and remains at the elevated level up to 4 M of NaCl. The rates of 2-propanol and 2,5-hexanediol oxidation are increased by more than 2fold after the addition of NaCl up to 1 M to the assay mixture and is retained at the increased level up to 4 M of NaCl |
726699 |
| 1.1.1.71 | Ca2+ |
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained |
740200 |
| 1.1.1.71 | KCl |
maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl |
726769 |
| 1.1.1.71 | KCl |
maximum activity in presence of 4 M KCl |
728288 |
| 1.1.1.71 | more |
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA |
726712 |
