EC Number   |
Metals/Ions |
Reference  |
|---|
   6.5.1.2 | NH4+ |
- |
1910 |
| 6.5.1.2 | NH4+ |
stimulates |
1910 |
| 6.5.1.2 | Rb+ |
stimulates |
1910 |
| 6.5.1.2 | KCl |
stimulation by 300 mM KCl, in 10% w/v polyethylene glycol 6000 solution or 150-300 mM KCl in 15% polyethylene glycol 6000 solution. Stimulation of intermolecular blunt end ligation by 150-250 mM KCl in 15% polyethylene glycol 600 solution |
1970 |
| 6.5.1.2 | NaCl |
stimulation of intermolecular cohesive-end ligation by 200 mM NaCl, in 10% w/v polyethylene glycol 6000 solution, or 100-200 mM NaCl in 15% polyethylene glycol 6000 solution. Stimulation of intermolecular blunt end ligation by 100-150 mM NaCl in 15% polyethylene glycol 600 solution |
1970 |
| 6.5.1.2 | K+ |
activates at 1-150 mM |
1971 |
| 6.5.1.2 | Mg2+ |
optimal concentration: 5-9 mM |
1971 |
| 6.5.1.2 | Mn2+ |
optimal concentration: 3-6 mM |
1971 |
| 6.5.1.2 | NH4+ |
activates at 1-150 mM |
1971 |
| 6.5.1.2 | Zn2+ |
zinc binding motif, Cys437, Cys440, Cys455, Cys460 |
649444 |
