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EC Number
Metals/Ions
Commentary
Reference
Mg2+
Mg2+ or Mn2+ required
Mn2+
Mg2+ or Mn2+ required
Mg2+
magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms
Mg2+
activates, optimum: 5-10 mM
Mn2+
activates, showing slightly higher activity than Mg2+
Mg2+
the active site contains two metal ions, consistent with the two-magnesium ion catalytic mechanism
Mg2+
L1 ligase is an obligate metalloenzyme that is highly specific for Mg2+. It is selected in the presence of 60 mM MgCl2 and functions optimally in Mg2+ concentrations as high as 100 mM
Mg2+
dependent on
Mn2+
the enzyme prefers manganese as the metal cofactor for nick sealing
Mg2+
enzyme activity is optimal at 5 mM Mg2+
<< < Results 11 - 20 of 22 > >>