Mg2+
activates, optimum: 5-10 mM
Mg2+
divalent cation required
Mg2+
enzyme activity is optimal at 5 mM Mg2+
Mg2+
Km: 0.4 mM, inhibition above 10 mM; required
Mg2+
L1 ligase is an obligate metalloenzyme that is highly specific for Mg2+. It is selected in the presence of 60 mM MgCl2 and functions optimally in Mg2+ concentrations as high as 100 mM
Mg2+
magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms
Mg2+
Mg2+ or Mn2+ required
Mg2+
nucleotidyl transfer Mg2+ dependent