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EC Number
Metals/Ions
Commentary
Reference
Mn2+
presence of Mn2+ restores activity after incubation with EDTA
more
enzyme does not require tightly bound metal ions for activity, they are not released even after extensive dialysis at pH 2.4
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His12, Gln65, His91 and Glu141 provide a binding site for a divalent metal ion, as shown by binding of Co2+
more
no activation of enzyme with Mg2+, Ni2+
Ni2+
activates; the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+)
Ni2+
increases activity to a smaller extent than Co2+
Zn2+
increases activity to a smaller extent than Co2+
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