EC Number   |
Metals/Ions |
Reference  |
|---|
    2.8.1.6 | Iron |
the as-isolated form of enzyme contains an air-stable [2Fe-2S]2+ center. Enzyme can additionally accomodate an air-sensitive [4Fe-4S]2+ center which is generated by incubation under anaerobic conditions with Fe2+ and S2-. With respect to iron cluster content and characteristics, mutants N151A, H152A, N153A, D155A are similar to wild-type |
672076 |
| 2.8.1.6 | Iron |
[2Fe-2S]2+ cluster coordinated by C97, C128, C188 and R260. Wild-type enzyme contains 1.6 iron atoms per monomer |
672097 |
| 2.8.1.6 | Iron |
the [2Fe-2S]2+ cluster is implicated in the sulfur insertion step |
672142 |
| 2.8.1.6 | Iron |
Fe/S cluster assembly of biotin synthase strongly depends on Isu1 and Isu2 proteins |
673421 |
| 2.8.1.6 | Iron |
Fe/S cluster assembly on Bio2 strongly depends on the Isu1 and Isu2 proteins, Isa proteins are crucial for the in vivo function of biotin synthase but not for the de novo synthesis of its Fe/S clusters. |
673421 |
| 2.8.1.6 | Iron |
BioB appears to be resistant to degradation and capable of multiple turnovers only under high-iron conditions that favor repair of the FeS clusters, a process most likely mediated by the Isc or Suf iron-sulfur cluster assembly systems. |
690617 |
| 2.8.1.6 | Iron |
loss of the FeS clusters results in decreased thermal stability and apparent localized unfolding of BioB, particularly in the regions around Arg168 and Arg245, but not global unfolding |
690617 |
| 2.8.1.6 | Iron |
the [2Fe-2S]2+ cluster is both the sulfur-donating substrate and the sulfur-oxidizing cofactor |
690617 |
