Mn2+
optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+; strict requirement
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enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+
Ca2+
activates, 48% as effective as Mn2+
Co2+
activates, 52% as effective as Mn2+
Mn2+
requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
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not activated by Mg2+, Zn2+, Cu2+ or Cd2+
Ca2+
about one third of the activity compared to Mn2+, recombinant enzyme
Mg2+
about one third of the activity compared to Mn2+, recombinant enzyme
Mn2+
required for activity, recombinant enzyme
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