EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
6.3.4.21 | 0.00022 |
- |
5-phospho-alpha-D-ribose 1-diphosphate |
+/-0.07 microM, in presence of 1 mM NAD+, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification |
680797 |
6.3.4.21 | 0.00025 |
- |
5-phospho-alpha-D-ribose 1-diphosphate |
+/-0.06 microM, 20 ng enzyme, 40 microM nicotinamide, 0.14-1 microM PRPP, 15 min, 37°C, TLC-based quantification |
680797 |
6.3.4.21 | 0.0005 |
- |
nicotinate |
in the presence of ATP |
637752 |
6.3.4.21 | 0.0008 |
0.0015 |
nicotinate |
- |
637757 |
6.3.4.21 | 0.001 |
- |
nicotinate |
- |
637749 |
6.3.4.21 | 0.0015 |
- |
nicotinate |
pncB gene, in the coupled reaction |
637761 |
6.3.4.21 | 0.002 |
- |
nicotinate |
- |
637748 |
6.3.4.21 | 0.0127 |
- |
nicotinic acid |
+/-3.6 microM, in presence of 1 mM NAD+, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification |
680797 |
6.3.4.21 | 0.013 |
- |
5-phospho-alpha-D-ribose 1-diphosphate |
reduced to 0.002 mM in the presence of ATP |
637755 |
6.3.4.21 | 0.0155 |
- |
nicotinic acid |
+/-3.4 microM, 80 ng enzyme, 10-70 microM nicotinamide, 0.3 mM PRPP, 30 min, 37°C, TLC-based quantification |
680797 |