EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.2.99.B1 | -999 |
- |
intact apurinic/apyrimidinic site |
similar to that of preincised apurinic/apyrimidinic site |
692941 |
4.2.99.B1 | -999 |
- |
more |
kinetic analysis of dRP lyase activity of the C-terminal domain of Rev1 using a 34-bp duplex DNA substrate. The substrate contained an 18mer DNA strand with 6-FAM at the 3'- end and 5'-RP flap at the margin of a single-nucleotide gap |
748781 |
4.2.99.B1 | 0.00021 |
- |
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex |
NEIL1 |
692334 |
4.2.99.B1 | 0.00045 |
- |
28-mer deoxyoligonucleotide with a 5' uracil residue |
pretreated with Eschericia coli uracil N-glycosylase, with concentrations 0.1-2.0 microMol, kcat/Km: 0.24 microMol/min |
692962 |
4.2.99.B1 | 0.0005 |
- |
DNA with a 5'-incised apurinic/apyrimidinic site at position 21 |
dRP lyase activity of beta-pol. To quantify the kinetic parameters for the release of deoxyribose phosphate from a preincused apurinic/apyrimidinic site-containing DNA, deoxyribose phosphate is examined as a function of apurinic/apyrimidinic site concentration. The catalytic efficiency results in a kcat/Km: 0.00015 mM/s. The catalytic efficiency of the 8-kDa amino-terminal domain is 7fold lower than that of the intact 39-kDa beta-pol |
692941 |
4.2.99.B1 | 0.0006 |
- |
preincised apurinic/apyrimidinic DNA |
pH 7.4, 37°C, in presence of 0.2 mM EDTA |
694925 |
4.2.99.B1 | 0.0018 |
- |
mimic of a preincised DNA duplex with a 5'dRP at the site of the nick |
pH 7.5, 37°C |
694866 |
4.2.99.B1 | 0.0022 |
- |
25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex |
NEIL2 |
692334 |
4.2.99.B1 | 0.007 |
- |
preincised apurinic/apyrimidinic DNA |
recombinant protein, pH 7.4, 37°C, in presence of 0.2 mM EDTA |
694925 |
4.2.99.B1 | 0.009 |
- |
35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15 |
full-length 290-kDa Pol theta, pH 7.5, 37°C, kcat/Km: 0.047 microM/min |
694467 |