EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.2.1.114 | -999 |
- |
more |
steady-state kinetics |
693150 |
4.2.1.114 | 0.022 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
wild type enzyme |
702368 |
4.2.1.114 | 0.022 |
- |
cis-homoaconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 0.03 |
- |
(1E)-pent-1-ene-1,2,5-tricarboxylic acid |
wild type enzyme |
702368 |
4.2.1.114 | 0.03 |
- |
cis-homo2aconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 0.036 |
- |
(1E)-hex-1-ene-1,2,6-tricarboxylic acid |
wild type enzyme |
702368 |
4.2.1.114 | 0.036 |
- |
cis-homo3aconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 0.135 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
mutant enzyme R26K |
702368 |
4.2.1.114 | 0.175 |
- |
cis-homo4aconitate |
pH 9.0, 60°C, recombinant enzyme, hydrolase reaction |
693150 |
4.2.1.114 | 0.22 |
- |
but-1-ene-1,2,4-tricarboxylic acid |
mutant enzyme R26V |
702368 |