EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    3.5.5.7 | -999 |
- |
more |
kinetics |
209747 |
| 3.5.5.7 | 0.4 |
- |
3-Cyanopyridine |
pH 7.0, 30°C |
756205 |
| 3.5.5.7 | 0.47 |
- |
m-tolunitrile |
mutant Y142V, pH 7.5, 25°C |
685014 |
| 3.5.5.7 | 0.49 |
- |
Benzonitrile |
mutant Y142V, pH 7.5, 25°C |
685014 |
| 3.5.5.7 | 0.61 |
- |
m-tolunitrile |
mutant Y142A, pH 7.5, 25°C |
685014 |
| 3.5.5.7 | 0.688 |
- |
adiponitrile |
mutant enzyme Y142F |
685014 |
| 3.5.5.7 | 0.69 |
- |
adiponitrile |
mutant Y142F, pH 7.5, 25°C |
685014 |
| 3.5.5.7 | 0.75 |
- |
potassium cyanide |
pH 7.0, 37°C, partially purified enzyme |
209747 |
| 3.5.5.7 | 0.82 |
- |
2-Cyanopyridine |
mutant Y142A, pH 7.5, 25°C |
685014 |
| 3.5.5.7 | 0.85 |
- |
adiponitrile |
mutant K9A, pH 7.5, 25°C |
685014 |
