EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.5.4.9 | -999 |
- |
more |
kinetics |
654529 |
3.5.4.9 | -999 |
- |
more |
the His-tag does not alter the activity and kinetics of the enzymes |
654797 |
3.5.4.9 | -999 |
- |
more |
the overall forward reaction from mythylene- to formyltetrahydrofolate shows a hydride transfer kinetic isotope effect, whereas the the overall reverse reaction does not |
701402 |
3.5.4.9 | 0.0045 |
- |
5,10-methenyltetrahydrofolate |
pH 7.6, 30°C, mutant enzyme G122D |
744335 |
3.5.4.9 | 0.009 |
- |
10-formyltetrahydrofolate |
pH 7.0, 23°C |
654529 |
3.5.4.9 | 0.009 |
- |
5,10-methenyltetrahydrofolate |
pH 7.0, 23°C |
654529 |
3.5.4.9 | 0.015 |
- |
5,10-methenyltetrahydrofolate |
pH 7.6, 30°C, mutant enzyme D121A |
744335 |
3.5.4.9 | 0.016 |
- |
10-formyltetrahydrofolate |
reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate |
654797 |
3.5.4.9 | 0.0164 |
- |
(6R,S)-methenyltetrahydrofolate |
- |
209736 |
3.5.4.9 | 0.018 |
- |
10-formyltetrahydrofolate |
reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate |
654797 |