EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.5.1.99 | -999 |
- |
more |
Michaelis-Menten kinetics, overview |
718570 |
3.5.1.99 | 0.0018 |
- |
anandamide |
liver microsomes, pH 7.3, 37°C |
718570 |
3.5.1.99 | 0.0024 |
- |
anandamide |
liver microsomes, pH 7.3, 37°C |
718570 |
3.5.1.99 | 0.0033 |
- |
anandamide |
liver mitochondria, pH 7.3, 37°C |
718570 |
3.5.1.99 | 0.007 |
- |
oleamide |
pH 9.0, mutant enzyme S218A, FAAH protein lacking its N-terminal 39 amino acids is used. This modification removes the predicted N-terminal transmembrane domain of FAAH, the deletion of which is previously found to leave catalytic properties of FAAH unaltered, while at the same time facilitating the purification |
690872 |
3.5.1.99 | 0.00843 |
- |
N-(6-methoxypyridin-3-yl)decanamide |
pH 8.0, 30°C |
752472 |
3.5.1.99 | 0.009 |
- |
oleoyl methyl amide |
pH 9.0, wild-type enzyme |
690871 |
3.5.1.99 | 0.011 |
- |
oleamide |
pH 9.0, wild-type enzyme, FAAH protein lacking its N-terminal 39 amino acids is used. This modification removes the predicted N-terminal transmembrane domain of FAAH, the deletion of which is previously found to leave catalytic properties of FAAH unaltered, while at the same time facilitating the purification |
690872 |
3.5.1.99 | 0.012 |
- |
oleamide |
pH 9.0, recombinantly expressed FAAH protein lacking the N-terminal transmembrane domain |
209111 |
3.5.1.99 | 0.012 |
- |
oleoyl p-nitroanilide |
pH 9.0, mutant enzyme K142A |
690871 |