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EC Number KM Value [mM] KM Value Maximum [mM] Substrate Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0041 - tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C 694073
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0041 - tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin - 694073
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0041 - tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin wild type enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C 732674
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0052 - tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin mutant enzyme W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C 732674
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.00613 - Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C 718252
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0067 - tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin mutant enzyme H61N/W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C 732674
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0096 - Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C 718252
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0098 - tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C 718252
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0106 - tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C 718252
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.B620.0117 - Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C 718252
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