EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.22.61 | -999 |
- |
more |
stopped-flow and steady-state kinetics |
717272 |
3.4.22.61 | 0.004 |
- |
acetyl-DEVD-7-amido-4-methylcoumarin |
pH 7.0 |
647424 |
3.4.22.61 | 0.0045 |
- |
Ac-IETD-4-methylcoumarin 7-amide |
pH 7.2, 25°C |
717272 |
3.4.22.61 | 0.007 |
- |
acetyl-DEVD-7-amido-4-methylcoumarin |
pH 7.5 |
647424 |
3.4.22.61 | 0.0206 |
- |
acetyl-IETD-7-amido-4-fluoromethylcoumarin |
cleaved caspase-8, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate |
663939 |
3.4.22.61 | 0.0293 |
- |
acetyl-IETD-7-amido-4-fluoromethylcoumarin |
non-cleavable caspase-8 + 0.001 mM FLIP D297A, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate |
663939 |
3.4.22.61 | 0.0313 |
- |
acetyl-IETD-7-amido-4-fluoromethylcoumarin |
non-cleavable caspase-8 + 0.001 mM FLIP, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate |
663939 |
3.4.22.61 | 0.0552 |
- |
acetyl-IETD-7-amido-4-fluoromethylcoumarin |
cleaved caspase-8, 37°C, pH 7.4 |
663939 |
3.4.22.61 | 0.066 |
- |
acetyl-IETD-4-nitroanilide |
pH 7.5, 37°C |
647441 |