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EC Number KM Value [mM] KM Value Maximum [mM] Substrate Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - hyaluronan kinetic measurement on hyaluronan substrates with sizes ranging from 90 to 752 kDa. Km-values: 0.87-0.91 mg/ml. A simple and sensitive method is developed for kinetic measurement of recombinant human hyaluronidase PH20 (rHuPH20) on hyaluronan substrates with sizes ranging from 90 to 752 kDa. The method is based on 2-aminobenzamide labeling of hydrolyzed hyaluronan products combined with separation by size exclusion-ultra performance liquid chromatography coupled with fluorescence detection 749559
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - hyaluronic acid Km: 0.00848 mg/ml, at 37°C, pH 8.0 751278
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - hyaluronoic acid 0.48 mg/ml 171394
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - hyaluronoic acid 0.71 mg/ml 171402
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - hyaluronoic acid value in mg/ml 171394, 171402
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - more kinetics 649321
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - more Km (hyaluronan) (microgram/ml): 4.91, Vmax (hyaluronan): 2.02 U/min 701325
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.35-999 - more Km strongly decreases with increasing HA chain sizes 699963
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.350.00091 - hyaluronic acid in 5 mM ammonium acetate, at 37°C and pH 5.0 681742
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.350.038 - hyaluronan Hyal1 wild-type 693261
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