EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.2.1.25 | -999 |
- |
more |
as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the KM-value of 0.3 mM is only an estimate |
654244 |
| 3.2.1.25 | 0.000083 |
- |
4-nitrophenyl-beta-mannopyranose |
pH 7.0, 37°C, mutant enzyme E215A |
665524 |
| 3.2.1.25 | 0.0028 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.015 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.017 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.079 |
- |
p-nitrophenyl-beta-D-mannopyranoside |
mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.088 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.089 |
- |
2,4-dinitrophenyl-beta-D-mannopyranoside |
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin |
680752 |
| 3.2.1.25 | 0.1 |
- |
Mannotriose |
pH 7.0, 37°C, wild-type enzyme |
665524 |
| 3.2.1.25 | 0.12 |
- |
p-nitrophenyl-beta-D-mannoside |
- |
136058 |
