EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    3.2.1.133 | -999 |
- |
more |
Km 131 mg/ml, starch as substrate, mutant W47A |
646803 |
| 3.2.1.133 | -999 |
- |
more |
Km 49.7 mg/ml, starch as substrate, pH 6.0, 60°C, wild-type, 1 M KCl |
646801 |
| 3.2.1.133 | -999 |
- |
more |
Km 5.34 mg mL-1, starch as substrate, pH 6.0, 60°C, hydrolysis, truncated mutant ThMA-DELTA124 |
646801 |
| 3.2.1.133 | -999 |
- |
more |
Km 61.0 mg/ml, starch as substrate, pH 6.0, 60°C, hydrolysis, wild-type |
646801 |
| 3.2.1.133 | -999 |
- |
more |
Km 7.72 mg/ml, starch as substrate, pH 6.0, 60°C, truncated mutant ThMA-DELTA124, 1M KCl |
646801 |
| 3.2.1.133 | -999 |
- |
more |
Km 73.5 mg/ml, starch as substrate, wild-type |
646803 |
| 3.2.1.133 | -999 |
- |
more |
lower affinity for binding of amylopectin but higher affinity for amylose in mutant enzymes demonstrated, sterospecific substrate binding properties of triple mutant enzyme determined by molecular modelling |
678795 |
| 3.2.1.133 | 0.05 |
- |
beta-cyclodextrin |
pH 6.0, 60°C, hydrolysis, variant DM |
646805 |
| 3.2.1.133 | 0.09 |
- |
beta-cyclodextrin |
pH 6.0, 60°C, hydrolysis, variant 4B74 |
646805 |
| 3.2.1.133 | 0.128 |
- |
beta-cyclodextrin |
- |
663819 |
