EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.11.1.1 | -999 |
- |
more |
kinetics |
650117 |
| 3.11.1.1 | -999 |
- |
more |
kinetics, activity of mutant D186A is too low to measure Km accurately |
650251 |
| 3.11.1.1 | 0.033 |
- |
phosphonoacetaldehyde |
25°C, pH 7.5, wild-type enzyme |
667598 |
| 3.11.1.1 | 0.033 |
- |
phosphonoacetaldehyde |
wild-type enzyme, mutant C22S, and mutant Y128F/C22S, pH 7.5, 25°C |
652569 |
| 3.11.1.1 | 0.033 |
- |
phosphonoacetaldehyde |
wild-type enzyme, pH 7.5, 25°C |
650117, 650251 |
| 3.11.1.1 | 0.035 |
- |
phosphonoacetaldehyde |
mutant Y128A, pH 7.5, 25°C |
652569 |
| 3.11.1.1 | 0.04 |
- |
phosphonoacetaldehyde |
- |
246529, 246531 |
| 3.11.1.1 | 0.045 |
- |
phosphonoacetaldehyde |
mutant Y128F, pH 7.5, 25°C |
652569 |
| 3.11.1.1 | 0.054 |
- |
phosphonoacetaldehyde |
mutant G185D/D190G, pH 7.5, 25°C |
650251 |
| 3.11.1.1 | 0.056 |
- |
phosphonoacetaldehyde |
triple mutant K121R/K146R/K192R, pH 7.5, 25°C |
650117 |
