EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.1.4.52 | -999 |
- |
more |
steady-state kinetics of wild-type and mutant RocRs, overview |
692857 |
| 3.1.4.52 | 0.0000029 |
- |
cyclic di-3',5'-guanylate |
pH and temperature not specified in the publication |
715164 |
| 3.1.4.52 | 0.00006 |
- |
cyclic di-3',5'-guanylate |
23°C, pH 7.0 |
680631 |
| 3.1.4.52 | 0.0002 |
- |
cyclic di-3',5'-guanylate |
mutant RocR enzyme D56N, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 |
704299 |
| 3.1.4.52 | 0.0003 |
- |
cyclic di-3',5'-guanylate |
mutant RocR enzyme E268Q, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 |
704299 |
| 3.1.4.52 | 0.0003 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 23°C, recombinant mutant E268Q |
692857 |
| 3.1.4.52 | 0.0003 |
- |
cyclic-di-GMP |
mutant enzyme E268Q, in 100 mM Tris buffer (pH 8.0) with 20 mM KCl and 25 mM MgCl2 |
692857 |
| 3.1.4.52 | 0.00042 |
- |
cyclic di-3',5'-guanylate |
presence of 0.004 mM GTP, pH 8.0, 30°C |
680630 |
| 3.1.4.52 | 0.0007 |
- |
cyclic di-3',5'-guanylate |
mutant RocR enzyme F297A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 |
704299 |
| 3.1.4.52 | 0.0013 |
- |
cyclic di-3',5'-guanylate |
pH 8.0, 23°C, recombinant mutant Q372A |
692857 |
