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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
3.1.3.75	0.003	-	O-Phosphoethanolamine	pH 6.7, 37°C, 2 mM Mg2+	654549
3.1.3.75	0.0114	-	phosphocholine	pH 6.7, 37°C, 2 mM Mg2+	654549
3.1.3.75	0.03	-	phosphocholine	at pH 5 and pH 7.4, without signal peptide	679380
3.1.3.75	0.03	-	phosphocholine	mutant T35S	679380
3.1.3.75	0.05	-	phosphocholine	at pH 5 and pH 7.4, with signal peptide	679380
3.1.3.75	0.44	-	phosphocholine	50 mM Hepes/NaOH buffer, pH 7.0, 37°C	714360
3.1.3.75	0.5	-	phosphocholine	at pH 5 and pH 7.4, without signal peptide	679380
3.1.3.75	0.5	-	phosphocholine	mutant T35S	679380
3.1.3.75	0.51	-	4-nitrophenyl phosphate	pH 5.0, 37°C, recombinant mutant Y82A/Y83A/Y84A	714357
3.1.3.75	0.56	-	4-nitrophenyl phosphate	pH 5.0, 37°C, recombinant mutant E43A	714357

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Release 2023.1 (January 2023)