EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.7.1.107 | -999 |
- |
more |
- |
640368, 640380 |
2.7.1.107 | -999 |
- |
more |
in presence of Triton X-100, used for purification, a biphasic dependency upon diacylglycerol is observed and the apparent Michaelis constant values for diacylglycerol decreases with decreasing Triton concentration |
640355 |
2.7.1.107 | -999 |
- |
more |
kinetics, follow a Michaelis-Menten mechanism in case of medium-chain diacylglycerols, and are strongly dependent on detergent concentration and sort used in the micelles in the assay in case of longer-chain substrates showing sigmoidal kinetics, overview |
661157 |
2.7.1.107 | -999 |
- |
more |
kinetics, recombinant wild-type and mutant enzymes |
661062 |
2.7.1.107 | -999 |
- |
more |
Km for dioleoylglycerol is 0.92 mol% |
640352 |
2.7.1.107 | -999 |
- |
more |
Km value for wild-type is 0.59 mol%, for mutant P32A 0.13 mol%, using substrate 1-stearoyl-2-oleoyl-sn-glycerol |
693667 |
2.7.1.107 | -999 |
- |
more |
Km value in mol% for substrate 1,2-dioleoylglycerol is 0.8 for isoform alpha, 0.52 for isoform zeta, pH 7.2, 30°C |
690889 |
2.7.1.107 | -999 |
- |
more |
Km value of wild-type, 2.35, mutant Y451, 2.07, mutant R457Q, 1.71 mol% 1-stearoyl-2-arachidonoyl-sn-glycerol, respectively, pH 7.2, 22°C |
722985 |
2.7.1.107 | -999 |
- |
more |
Km values of isozymes DGKzeta, DGKepsilon, and DGKalpha with different substrates in mol% |
661105 |
2.7.1.107 | -999 |
- |
more |
Km-value in mol% for wild-type 9.2, mutant C46A/C113A 4.9, mutant C46A/C113A/E28C 5.7, mutant C46A/C113A/A29C 7.4, mutant C46A/C113AA30C 27.2, mutant C46A/C113A/F31C 17.2, mutant C46A/C113A/R32C 12.9, mutant C46A/C113A/Q33C 12.1, mutant C46A/C113A/E34C 13.7 |
690881 |