EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   2.4.2.12 | 0.000005 |
- |
nicotinamide |
+/-0.000002 mM, in presence of 2 mM ATP the Km is 150-fold lowered, calculated from apparent KM by extrapolation to correct for inhibition by NAD+ in the continuous fluorimetric assay, pH 7.5, 200 microM PRPP, 2.8 nM NAMPT |
690900 |
| 2.4.2.12 | 0.000067 |
- |
nicotinamide |
- |
489738 |
| 2.4.2.12 | 0.000235 |
- |
nicotinamide |
+/-0.000017 mM, in presence of 2 mM phosphate, pH 7.5, 100 microM PRPP, 88 nM NAMPT, HPLC-based quantification |
690900 |
| 2.4.2.12 | 0.00051 |
- |
5-phosphoribosyl 1-diphosphate |
with 1 mM ATP, pH 7.5, 37°C |
723535 |
| 2.4.2.12 | 0.00063 |
- |
alpha-D-5-phosphoribosyl 1-diphosphate |
+/-0.00003 mM, in presence of 2 mM ATP the Km is lowered 10-fold, calculated from apparent KM by extrapolation to correct for inhibition by NAD+ in the continuous fluorimetric assay, pH 7.5, 125 nM nicotinamide, 2.2 nM NAMPT |
690900 |
| 2.4.2.12 | 0.000855 |
- |
nicotinamide |
+/-0.000022 mM, pH 7.5, 100 microM PRPP, 88 nM NAMPT, HPLC-based quantification |
690900 |
| 2.4.2.12 | 0.001 |
- |
nicotinamide |
in the presence of ATP |
489739 |
| 2.4.2.12 | 0.00122 |
- |
nicotinamide |
with 1 mM ATP, pH 7.5, 37°C |
723535 |
| 2.4.2.12 | 0.00124 |
- |
nicotinamide |
- |
489743 |
| 2.4.2.12 | 0.00127 |
- |
nicotinamide |
- |
489742 |
