EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.1.1.28 | -999 |
- |
more |
Km-value of mutant enzymes |
639451 |
2.1.1.28 | 0.00054 |
- |
3-trifluoromethylphenylethanolamine |
pH 8.0, 30°C |
733211 |
2.1.1.28 | 0.00055 |
- |
3-trifluoromethylphenylethanolamine |
wild type enzyme, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine |
675288 |
2.1.1.28 | 0.0008 |
- |
3,4-Dichlorophenylethanolamine |
- |
639439 |
2.1.1.28 | 0.0012 |
- |
S-adenosyl-L-methionine |
cosubstrate 3-trifluoromethylphenylethanolamine, pH 8.0, 30°C |
733211 |
2.1.1.28 | 0.0012 |
- |
S-adenosyl-L-methionine |
wild type enzyme, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine |
675288 |
2.1.1.28 | 0.0013 |
- |
S-adenosyl-L-methionine |
mutant E185Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C. |
672011 |
2.1.1.28 | 0.00132 |
- |
3,4-Dichlorophenylethanolamine |
enzyme form H-2 |
639432 |
2.1.1.28 | 0.0015 |
- |
anti-9-amino-6-(trifluoromethyl)benzonorbornene |
wild type enzyme, assay containing S-adenosyl-L-methionine and anti-9-amino-6-(trifluoromethyl)benzonorbornene |
675288 |
2.1.1.28 | 0.00159 |
- |
3,4-Dichlorophenylethanolamine |
enzyme form H-1 |
639432 |