EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.20.4.4 | -999 |
- |
arsenate |
pH 7.5, 37°C, at low substrate concentrations the Km-value for arsenate is 0.0008 mM. Above 1 mM arsenate, a second increase in rate with increasing substrate is observed, with an apparent Km of 2 mM arsenate |
639368 |
| 1.20.4.4 | -999 |
- |
more |
NADPH oxidation shows Michaelis-Menten kinetics with a Km of 1 microM AsO43- and an apparent Vmax of 200 nmol/min per mg of protein. At high substrate concentration (above 1 mM AsO43-), a secondary rise in the reaction rate is observed, with a Km of 2 mM and an apparent Vmax of 450 nmol/min per mg of protein |
639368 |
| 1.20.4.4 | 0.000066 |
- |
arsenate |
pH 7.5, 37°C, wild-type enzyme |
639370 |
| 1.20.4.4 | 0.000066 |
- |
arsenate |
wild-type |
639370 |
| 1.20.4.4 | 0.000134 |
- |
arsenate |
C15A mutant |
639370 |
| 1.20.4.4 | 0.000134 |
- |
arsenate |
pH 7.5, 37°C, mutant enzyme C15A |
639370 |
| 1.20.4.4 | 0.0008 |
- |
arsenate |
high affinity |
639368 |
| 1.20.4.4 | 0.006 |
- |
arsenate |
pH 7.6, 37°C, presence of 150 mM KCl |
732190 |
| 1.20.4.4 | 0.007 |
- |
arsenate |
pH 7.6, 37°C, presence of 50 mM K2SO4 |
732190 |
| 1.20.4.4 | 0.008 |
- |
arsenate |
pH 7.6, 37°C, presence of 150 mM NaCl |
732190 |
