EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.14.16.1 | -999 |
- |
L-phenylalanine |
wild-type, pH 7.3, 25°C, measurement by standard discontinuous PAH activity assay (HPLC and fluorimetric detection): Vmax: 0.495 micromol L-Tyr/min/mg (without L-Phe preincubation), 1.55 micromol L-Tyr/min/mg (with L-Phe preincubation), S0.5: 0.318 mM (without L-Phe preincubation), S0.5: 0.154 mM (with L-Phe preincubation) |
712471 |
| 1.14.16.1 | -999 |
- |
more |
binding constants for Fe2+ of wild-type and mutant enzymes, overview |
684717 |
| 1.14.16.1 | -999 |
- |
more |
kinetic analysis |
686642 |
| 1.14.16.1 | -999 |
- |
more |
kinetic and molecular modelling of sulfur-containing substrates, overview |
688016 |
| 1.14.16.1 | -999 |
- |
more |
kinetics, molecular dynamics simulations, structure-energetics calculations, and molecular interaction fields, overview |
673511 |
| 1.14.16.1 | -999 |
- |
more |
thermodynamics of iron nitrosyl formation |
674002 |
| 1.14.16.1 | -999 |
- |
more |
thermodynamics, overview |
687083 |
| 1.14.16.1 | -999 |
- |
more |
[S]0.5 (L-phenylalanine): 0.188 mM wild-type,0,109 mM mutant Q215K/N216Y, Vmax (wild-type): 1.9 micromol L-Tyr/min/mg, Vmax (mutant Q215K/N216Y): 6.6 micromol L-Tyr/min/mg, pH 7.0, 25°C |
710830 |
| 1.14.16.1 | 0.001 |
- |
5,6,7,8-tetrahydrobiopterin |
using L-phenylalanine as cosubstrate |
700235 |
| 1.14.16.1 | 0.002 |
- |
tetrahydrobiopterin |
native liver enzyme |
438731 |
