EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.1.1.17 | -999 |
- |
more |
kinetic isotope effects, overview |
685964 |
| 1.1.1.17 | -999 |
- |
more |
Michaelis-Menten kinetics |
741122 |
| 1.1.1.17 | -999 |
- |
more |
steady-state kinetics and kinetic mechanism, binding of D-mannitol 1-phosphate and NAD+ is random, whereas D-fructose 6-phosphate binds only after NADH has bound to the enzyme. Hydride transfer is rate-determining for D-mannitol 1-phosphate oxidation by AfM1PDH. AfM1PDH behaves kinetically as a fructose 6-phosphate reductase, overview |
722234 |
| 1.1.1.17 | 0.005 |
- |
NADH |
cosubstrate: D-fructose 6-phosphate |
389421 |
| 1.1.1.17 | 0.014 |
- |
NADH |
pH 10.0, 25°C, recombinant enzyme |
722234 |
| 1.1.1.17 | 0.016 |
- |
NADH |
cosubstrate: D-fructose 6-phosphate |
389423 |
| 1.1.1.17 | 0.016 |
- |
NADH |
pH 7.1, 25°C, recombinant enzyme |
685964 |
| 1.1.1.17 | 0.02 |
- |
NAD+ |
recombinant enzyme, pH 9.0, 30°C |
741122 |
| 1.1.1.17 | 0.03 |
- |
NADH |
higher affinity for NADH |
389415 |
| 1.1.1.17 | 0.038 |
- |
D-mannitol 1-phosphate |
cosubstrate: NAD+ |
389421 |
