EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.99.5 | -999 |
- |
more |
complex kinetics of formation of the reaction intermediate, overview |
727690 |
4.1.99.5 | -999 |
- |
more |
Michaelis-Menten kinetics |
749051 |
4.1.99.5 | -999 |
- |
more |
the rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction |
747074 |
4.1.99.5 | 0.2 |
- |
n-Heptanal |
recombinant mutant F150Y, pH and temperature not specified in the publication |
747409 |
4.1.99.5 | 0.244 |
- |
n-Heptanal |
pH 7.2, 22°C, recombinant enzyme with Fd/FNR system |
727215 |
4.1.99.5 | 0.3 |
- |
n-Heptanal |
recombinant wild-type enzyme, pH and temperature not specified in the publication |
747409 |
4.1.99.5 | 0.32 |
- |
n-Heptanal |
recombinant mutant L148R, pH and temperature not specified in the publication |
747409 |
4.1.99.5 | 0.33 |
- |
n-Heptanal |
recombinant mutant Q49H/F150Y, pH and temperature not specified in the publication |
747409 |
4.1.99.5 | 0.34 |
- |
n-Heptanal |
recombinant mutant L146T, pH and temperature not specified in the publication |
747409 |
4.1.99.5 | 0.34 |
- |
n-Heptanal |
recombinant mutant W178R, pH and temperature not specified in the publication |
747409 |