EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.99.5 | 0.035 |
- |
octadecanal |
pH 7, 30°C |
648391 |
4.1.99.5 | 0.18 |
- |
octadecanal |
pH 7, 37°C |
648392 |
4.1.99.5 | 0.065 |
- |
octadecanal |
25°C |
648393 |
4.1.99.5 | 0.244 |
- |
n-Heptanal |
pH 7.2, 22°C, recombinant enzyme with Fd/FNR system |
727215 |
4.1.99.5 | -999 |
- |
more |
complex kinetics of formation of the reaction intermediate, overview |
727690 |
4.1.99.5 | -999 |
- |
more |
the rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction |
747074 |
4.1.99.5 | 0.069 |
- |
n-nonanal |
pH 7.2, 37°C, recombinant wild-type enzyme |
747351 |
4.1.99.5 | 0.14 |
- |
n-nonanal |
pH 7.2, 37°C, recombinant mutant A121F |
747351 |
4.1.99.5 | 0.18 |
- |
n-octanal |
pH 7.2, 37°C, recombinant wild-type enzyme and mutant A121F |
747351 |
4.1.99.5 | 0.56 |
- |
n-Heptanal |
pH 7.2, 37°C, recombinant mutant A121F |
747351 |