EC Number   |
KM Value [mM]  |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   4.1.2.52 | -999 |
- |
more |
apparent Km values for Co2+ increase in the two mutants H45A and H45Q by about 800fold compared to the wild-type enzyme |
722258 |
| 4.1.2.52 | 0.006 |
- |
4-hydroxy-2-oxopentanoate |
Km (app) with Co2+, pH 8.0, 25°C |
721601 |
| 4.1.2.52 | 0.0175 |
- |
4-hydroxy-2-oxopentanoate |
Km (app) with Mn2+, pH 8.0, 25°C |
721601 |
| 4.1.2.52 | 0.05 |
- |
2-oxo-3-methyl-4-hydroxypentanoate |
wild type enzyme, at pH 8.0 and 25°C |
725493 |
| 4.1.2.52 | 0.16 |
- |
4-hydroxy-2-oxohexanoate |
pH 8.0, 25°C |
721601 |
| 4.1.2.52 | 0.289 |
- |
4-hydroxy-2-oxopentanoate |
Km (app) with Mg2+, pH 8.0, 25°C |
721601 |
| 4.1.2.52 | 0.35 |
- |
4-hydroxy-2-oxoheptane-1,7-dioate |
using H2O as solvent, in the presence of 0.5 mM Co2+, at pH 8.0 and 25°C |
725493 |
| 4.1.2.52 | 0.38 |
- |
(S)-4-hydroxy-2-oxopentanoate |
wild type enzyme, with 0.5 mM Co2+, at pH 8.0 and 25°C |
725493 |
| 4.1.2.52 | 0.38 |
- |
4-hydroxy-2-oxopentanoate |
pH 8.0, 25°C |
721601 |
| 4.1.2.52 | 0.38 |
- |
4-hydroxy-2-oxopentanoic acid |
wild-type, pH 8.0, 25°C |
722258 |
