EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate  |
Reference |
|---|
   3.4.22.69 | 0.306 |
- |
(Ala-Arg-Leu-Gln-NH)2-rhodamine |
rate of hydrolysis measured by change in absorbance at 496 nm |
668633 |
| 3.4.22.69 | 0.0017 |
- |
Abz-LQSGFRK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.023 |
- |
Abz-LQSGFRK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0005 |
- |
Abz-LYQPPQTSITSAVLQSGFRK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.011 |
- |
Abz-QTSITSAVLQSGFRK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0024 |
- |
Abz-SAVLQSGFRK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.016 |
- |
Abz-SAVLQSGFRK(Dnp)NH2 |
in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0013 |
- |
Abz-SAVLQSGFRKMAFPSGK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.002 |
- |
Abz-SAVLQSGFRKMAK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
| 3.4.22.69 | 0.0016 |
- |
Abz-SAVLQSGK(Dnp)NH2 |
in the presence of 1 M sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C |
717308 |
