EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.21.9 | 0.001 |
- |
Trypsinogen |
bovine guanidinated trypsinogen |
95572 |
3.4.21.9 | 0.0014 |
- |
human cationic trypsinogen |
37 °C, 100 mM Tris-HCl pH 8.0, 1mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase |
669370 |
3.4.21.9 | 0.0021 |
- |
A4-mutant trypsinogen |
37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase |
669370 |
3.4.21.9 | 0.00258 |
- |
GD4R-4-nitroanilide |
mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C |
732883 |
3.4.21.9 | 0.0045 |
- |
Trypsinogen |
bovine trypsinogen |
95571 |
3.4.21.9 | 0.0056 |
- |
Trypsinogen |
pH 5.6 |
95578 |
3.4.21.9 | 0.00629 |
- |
GD4R-4-nitroanilide |
wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C |
732883 |
3.4.21.9 | 0.007 |
- |
Trypsinogen |
- |
95572 |
3.4.21.9 | 0.007 |
- |
Trypsinogen |
bovine trypsinogen |
95578 |
3.4.21.9 | 0.0072 |
- |
human cationic trypsinogen |
25 °C, 28 mM sodium succinate (pH 5.6), 10 mM CaCl2 |
669370 |