EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate  |
Reference |
|---|
   3.3.2.6 | 0.47 |
- |
alanine-4-nitroanilide |
pH 7.5, 25°C |
647002 |
| 3.3.2.6 | 0.5 |
- |
alanine-4-nitroanilide |
pH 7.6, 22°C |
646992 |
| 3.3.2.6 | 1.3 |
- |
Arg-Ser-Arg |
wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
| 3.3.2.6 | 1.9 |
- |
L-Ala-p-nitroanilide |
mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
| 3.3.2.6 | 1.9 |
- |
L-Ala-p-nitroanilide |
wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
| 3.3.2.6 | 3 |
- |
L-Ala-p-nitroanilide |
mutant enzyme D375A, 500 mM KCl in 250 mM Tris buffer (pH 7.5) |
697288 |
| 3.3.2.6 | 0.31 |
- |
L-alanine 2-naphthylamide |
mutant N362L, 25°C, pH not specified in the publication |
714325 |
| 3.3.2.6 | 0.339 |
- |
L-alanine 2-naphthylamide |
mutant W365L, 25°C, pH not specified in the publication |
714325 |
| 3.3.2.6 | 0.4 |
- |
L-alanine 2-naphthylamide |
mutant N362Q, 25°C, pH not specified in the publication |
714325 |
| 3.3.2.6 | 0.618 |
- |
L-alanine 2-naphthylamide |
mutant N362E, 25°C, pH not specified in the publication |
714325 |
