EC Number   |
KM Value [mM]  |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   2.1.1.56 | 0.002 |
- |
S-adenosyl-L-methionine |
- |
485462 |
| 2.1.1.56 | 0.003 |
- |
S-adenosyl-L-methionine |
intact mRNA capping enzyme and isolated methyltransferase domain |
485467 |
| 2.1.1.56 | 0.006 |
- |
S-adenosyl-L-methionine |
- |
674701 |
| 2.1.1.56 | 0.00745 |
- |
GTP |
at pH 7.3 and 25°C |
758535 |
| 2.1.1.56 | 0.0075 |
- |
S-adenosyl-L-methionine |
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.008 |
- |
S-adenosyl-L-methionine |
mutant enzyme K607A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.0095 |
- |
S-adenosyl-L-methionine |
mutant enzyme N570A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.023 |
- |
G(5')pppR-RNA |
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.031 |
- |
G(5')pppR-RNA |
mutant enzyme K607A, in 50 mM Tris-HCl (pH 7.5), 5 mM dithiothreitol, at 30°C |
706721 |
| 2.1.1.56 | 0.033 |
- |
GTP |
intact mRNA capping enzyme and isolated methyltransferase domain |
485467 |
