EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.8.4.9 | 0.02 |
- |
3'-phosphoadenosine 5'-phosphosulfate |
- |
687585 |
1.8.4.9 | 0.031 |
- |
3'-phosphoadenosine-5'-phosphosulfate |
assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
1.8.4.9 | 0.011 |
- |
5'-adenylyl sulfate |
- |
667746 |
1.8.4.9 | 0.0112 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: dithiothreitol |
689431 |
1.8.4.9 | 0.0174 |
- |
5'-adenylyl sulfate |
30°C, pH 8.5, cosubstrate: glutathione |
689431 |
1.8.4.9 | 0.00057 |
- |
5'-adenylylsulfate |
assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C |
667703 |
1.8.4.9 | 0.0025 |
- |
5'-adenylylsulfate |
with glutathione as reductant |
288642 |
1.8.4.9 | 0.0033 |
- |
5'-adenylylsulfate |
in the presence of 0.001 mM thioredoxin |
669891 |
1.8.4.9 | 0.0038 |
- |
5'-adenylylsulfate |
with DTT as reductant |
288642 |
1.8.4.9 | 0.0052 |
- |
5'-adenylylsulfate |
in the presence of 0.01 mM thioredoxin |
669891 |