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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
1.5.3.5
0.000082
-
FAD
-
392436
1.5.3.5
0.000114
-
(S)-nicotine
pH and temperature not specified in the publication
764183
1.5.3.5
0.011
-
(S)-6-hydroxynicotine
pH 7.0, 25°C, mutant enzyme K287M
741943
1.5.3.5
0.019
-
(S)-6-hydroxynicotine
pH 7.0, 40°C
741686
1.5.3.5
0.02
-
(S)-6-hydroxynicotine
pH and temperature not specified in the publication
712752
1.5.3.5
0.02
-
6-hydroxy-L-nicotine
-
392436
,
392440
1.5.3.5
0.06
-
(S)-6-hydroxynicotine
wild-type, pH 7.4, 22°C
764029
1.5.3.5
0.064
-
(S)-6-hydroxynornicotine
pH 7.0, 25°C, wild-type enzyme
741943
1.5.3.5
0.13
-
(S)-6-hydroxynicotine
pH 7.0, 25°C, wild-type enzyme
741943
1.5.3.5
0.19
-
O2
pH 7.0, 25°C, mutant enzyme Y311F, cosubstrate: (S)-6-hydroxynicotine
741943
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