EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.2.4.1 | 0.016 |
- |
2-oxobutanoate |
pH 6.7, 30°C |
736649 |
1.2.4.1 | 0.149 |
- |
2-oxovalerate |
pH 6.7, 30°C |
736649 |
1.2.4.1 | 0.73 |
- |
E2p lipoyl domain |
wild type enzyme, at pH 7.0 and 30°C |
725205 |
1.2.4.1 | 0.024 |
- |
lipoyl domain |
acyl group transfer reaction, recombinant pyruvate dehydrogenase with N-terminal deletion |
348990 |
1.2.4.1 | 0.027 |
- |
lipoyl domain |
acyl group transfer reaction, recombinant wild-type pyruvate dehydrogenase |
348990 |
1.2.4.1 | 1 |
- |
Mg2+ |
- |
348936 |
1.2.4.1 | -999 |
- |
more |
detailed kinetic and thermodynamic analysis |
672086 |
1.2.4.1 | -999 |
- |
more |
kinetics |
655474 |
1.2.4.1 | -999 |
- |
more |
sigmoidal dependence on the concentration of pyruvate, Hill-coefficient: 1.85 |
348960 |
1.2.4.1 | -999 |
- |
more |
with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 |
654462 |