EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   5.4.99.64 | 0.433 |
- |
(S)-3-hydroxybutanoyl-CoA |
pH 6.6, 30°C, mutant enzyme, I90A (mutation in subunit HcmA) |
738677 |
| 5.4.99.64 | 0.517 |
- |
2-hydroxy-2-methylpropanoyl-CoA |
mutant I90L, pH 6.6, 30°C |
738677 |
| 5.4.99.64 | 0.517 |
- |
2-hydroxy-2-methylpropanoyl-CoA |
pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) |
738677 |
| 5.4.99.64 | 0.55 |
- |
isobutanoyl-CoA |
pH 6.6, 30°C, wild-type enzyme |
736409 |
| 5.4.99.64 | 0.663 |
- |
(S)-3-hydroxybutanoyl-CoA |
mutant I90L, pH 6.6, 30°C |
738677 |
| 5.4.99.64 | 0.663 |
- |
(S)-3-hydroxybutanoyl-CoA |
pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) |
738677 |
| 5.4.99.64 | 0.977 |
- |
2,2-dimethylpropanoyl-CoA |
pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) |
738677 |
| 5.4.99.64 | 1.66 |
- |
(R)-3-hydroxybutanoyl-CoA |
pH 6.6, 30°C, wild-type enzyme |
736409, 738677 |
| 5.4.99.64 | 1.66 |
- |
(R)-3-hydroxybutanoyl-CoA |
wild-type, pH 6.6, 30°C |
738677 |
| 5.4.99.64 | 1.76 |
- |
(S)-3-hydroxybutanoyl-CoA |
mutant I90V, pH 6.6, 30°C |
738677 |
