EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.3.3 | 0.32 |
- |
N-acetylneuraminate |
wild type enzyme, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 0.35 |
- |
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid |
pH 7.5, 25°C, mutant enzyme E192V |
666805 |
| 4.1.3.3 | 0.39 |
- |
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide |
mutant enzyme E192N |
663630 |
| 4.1.3.3 | 0.39 |
- |
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid |
pH 7.5, 25°C, mutant enzyme E192N |
666805 |
| 4.1.3.3 | 0.4 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192N mutant protein, pH 7.7.5, 25°C |
715921 |
| 4.1.3.3 | 0.4 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192V mutant protein, pH 7.5, 25°C |
715921 |
| 4.1.3.3 | 0.4 |
- |
N-acetylneuraminic acid |
pH 7.2, Y133F mutant |
651103 |
| 4.1.3.3 | 0.5 |
- |
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid |
pH 7.5, 25°C, mutant enzyme E192S |
666805 |
| 4.1.3.3 | 0.5 |
- |
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid |
E192H mutant protein, pH 7.5, 25°C |
715921 |
| 4.1.3.3 | 0.5 |
- |
pyruvate |
mutant enzyme P193Y, at pH 7.0 and 37°C |
749150 |
